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BIO 599 Graduate
Independent Study
Jeffrey Brentson
Advisor: Dr. Edwin Wong
Fall 2006
Nature of
the project;
The independent study project will be creating a research experience for
high school students dealing with the Barcode of Life initiative (an international
effort to use the COX 1 gene as a ³barcode² to identify eukaryotic organisms).
Methods used
in the project
The teacher, students and Dr. Wong will work together to determine the
best method to isolate and amplify fish DNA. The goal then will be to
try to sequence a variety of local marine fishes and input the sequences
into the international Barcode of Life database. All of the students in
the teacherıs class will be exposed to the isolation of the DNA, amplification
of the COX 1 gene DNA, the sequencing data and interpretation of the data.
Only a few of the students will take part in the use of the DNA sequencer
due to the distance from WCSU.
Criteria
to evaluate the student's performance
The evaluation criteria will be whether or not the teacher and students
have successfully sequenced the DNA, input the data on the BOL website
and generated a report and interpretation of their results. Results will
also be presented at a poster presentation in May 2007, sponsored by the
WestConn Institute for Science Teacher Research (WISTR). Most of the data
collection and reporting should be finished by February of 2007.
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